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After Dorner`s Endospore Staining procedure, the endospores appear dark pink- because of safranine and are surrounded by a colourless (or sometimes faint pink if the stain is not properly washed off) vegetative cell. The background is stained black or gray because of nigrosine.
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You're reading In the Dorner endospore stain, a smear covered with carbolfushin is steamed, then decolorized with acid-alcohol and countersigned with nigrosin. Describe the microscope appearance after this procedure.
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