Why is the complementarity of nucleotides an integral part of biotechnology? How is it involved?
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ANSWERS: 1
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"An important biochemical strategy for joining together DNA segments depends on the ability of nucleotides in a single strand of DNA to pair with complementary nucleotides on the other strand to form a DNA duplex. The A's and G's pair with C's and T's, as Watson and Crick had found some years earlier. The late 1960's work in the laboratory of Gobind Khorana showed that short segments of synthetic single strand pieces of DNA could be linked in test tubes using pairing of overlapping nucleotides and building up segments of DNA in this way. DNA ligase, an enzyme that repairs breaks in DNA, can seal the molecules together. However, construction of overlapping complementary DNA ends, as shown here by the stepwise biosynthetic addition of single nucleotides, was both technically difficult and laborious." Source and further information: http://bancroft.berkeley.edu/ROHO/projects/biosci/symposium/cohen/text.html
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