by 
Anonymous
ANSWERS: 1
Non Contact Infrared Thermometer -- $19.99
While Supplies Last
13deals
.png)
Ad
-
It depends on what protein the blot is being used to detect, and the protocol being used. Minimum time is 4-5 hours, including materials preparation and stripping the blot afterwards. Some protocols strip the blot overnight, however. (This step is necessary to make the tagged proteins visible in isolation.) To a large degree it also depends on the availability of the antibody which is being used to tag the target protein, though. For a standard medical test you would expect them to have a stock of antibody on hand, so it shouldn't add much time. If they are testing for something unusual, however, it may take up to a month to manufacture adequate levels of antibody...or it may be something which can be obtained elsewhere in a few days to a week or so. It just depends. Not all Westerns are run identically.
RELATED QUESTIONS
Copyright 2023, Wired Ivy, LLC