Is the disk-dilution technique measuring bacteriostatic or bactericidal activity? Briefly explain?
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ANSWERS: 1
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1) "This disk diffusion technique does not allow us to distinguish between bacteriostatic and bactericidal effects of the antimicrobial chemicals." Source and further information: http://www.ncrrsepa.org/Ed/BSEP/MicrobiologyLab/Micro_lab_protocols.pdf 2) "Differentiation between bacteriostatic and bacteriocidal effects. In order to distinguish the bacteriocidal effect of polyP from a bacteriostatic effect of polyP, viable cell counts of B. cereus cultures that were in different growth phases and were treated with 0.1% JOHA HBS were determined. Six 50-ml portions of broth were inoculated with 105 CFU/ml. After 0, 2, 4, 6, and 24 h, 0.1% JOHA HBS was added to each sample, the cultures were incubated, and the viable cells were enumerated by plating at different times. To determine if the bacteriostatic effect could be reversed, 5 mM Ca2+ and 5 mM Mg2+ were added to cultures which had been preincubated with 0.1% JOHA HBS for 24 h, and the increases in viable cell number were monitored by surface plating for the next 8 h." Source and further information: http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=99724
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