by Kiki on January 15th, 2008

Kiki

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Can you determine whether a broth culture is pure (all one species) by visually inspecting it without a microscope? With an agar deep culture? An agar slant culture?

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  • by Smokin' Pope on January 16th, 2008

    Smokin' Pope

    broth: generally no. but if you're culturing something that grows whitish, like standard e. coli, and something green is floating around then that's obviously not pure. but if there's another whitish bacteria, you can't tell.

    deep: possibly, if your culture only grows on the surface and you're contaminated with something that grows in the bottom (anaerobic).

    slant: possibly also. if you see colonies of your whitish bacteria and some colonies that don't look the same.

    none of them are that good to determine if you have a contamination without staining or microscopy

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Can you determine whether a broth culture is pure by visually inspecting it without a microscope
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