by Anonymous on October 18th, 2007

Anonymous

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In the Dorner endospore stain, a smear covered with carbolfuchsin is steamed, then decolorized with acid-alcohol and counterstained with nigrosin. Describe the microscopic appearance after this precedure.

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  • by Emily_P9908 on February 9th, 2011

    Emily_P9908

    As far as I can tell, the carbolfuchsin, when steamed, would stain the endospore green, the decolorizing would leave the cell clear, and the counter stain of nigrosin, which is used as a negative stain, would stain the background dark.

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In the dorner endospore stain a smear covered with
Dorner endospore stain
Dorner stain
In the dorner endospore stain a smear covered with carbolfuchsin
In the dorner endospore stain a smear covered with carbolfuchsin is steamed