How do autoclaves work?

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Hospitals and laboratories use autoclaves to sterilize equipment, surgical tools and to kill bacteria and other organisms. Contents inside an autoclave can reach up to 270 degrees F, as high pressured steam circulates through the device.

Sterilization Methods

An autoclave may use a combination of high temperatures, pressurization and vacuuming to sterilize equipment, according to the UC Berkley Office of Environment, Health & Safety.

Duration

The full sterilization cycle of an autoclave takes approximately 30 minutes, according to the University of Massachusetts Amherst.

Maintenance

The University of Massachusetts Amherst recommends annual maintenance inspections of autoclave equipment and quarterly spore tests to ensure proper sterilization.

Sterilization Confirmation

When using an autoclave, an indicator strip and special tape change color once sterilization is complete, confirming that the appropriate temperature range was reached.

Safety Equipment

People operating an autoclave should use gloves designed for high heat exposure, protective eye goggles and in cases of liquid sterilization, rubber boots and an apron, according to the UC Berkley Office of Environment, Health & Safety.

Source:

UC Berkley Office of Environment, Health & Safety: Using Autoclaves Safely

University of Massachusetts Amherst Environmental Health and Safety: Autoclaved Waste

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