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<h4 class="dechead">On One Hand: Yes You Can
For both the Xbox 360 and PlayStation 3, the Guitar Hero: World Tour guitar is fully compatible with all Rock Band content, including Rock Band, Rock Band 2 and the Rock Band AC/DC Track Pack.
On the Other: Not Automatically
The Guitar Hero: World Tour guitar does not work with Rock Band on either the Nintendo Wii or PlayStation 2 platforms. Furthermore, because the Guitar Hero: World Tour guitar was not designed specifically for Rock Band, it can be quite a chore to properly configure the guitar for the game.
Bottom Line
Depending on the platform on which you want to play Rock Band with your Guitar Hero: World Tour guitar, you will either find success or failure. But even for the systems that do allow the instrument crossover, using it in-game can be quite tricky.
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