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by Kris102009 on December 1st, 2009

Kris102009

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In the Dorner endospore stain, if a smear is covered with carbolfuchsin, steamed, decolorized with acid alcohol, and counterstained with nigrosin, how will it appear microscopically?

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Answers. 2 helpful answers below.

  • by Chris_S7528 on September 28th, 2011

    Chris_S7528

    Carbolfuchsin will stain the endospore and the vegetative cell pink, the acid alcohol will wash the stain out of the cell but not the spore, then the nigrosin counterstain will stain the background dark as in a negative stain. So, you will be left with red endospores, clear or unstained vegetative cells and a dark background.

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  • by Jim in a Nautilus COAT on December 1st, 2009

    Jim in a Nautilus COAT

    Ha! I'm not a microbiologist, but I play one on Google.

    http://answers.yahoo.com/question/index?qid=20080219125408AAg4yaB

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